Beta-Hydroxy-Beta-Methylbutyrate or Calcium HMB is a metabolite of the essential amino acid leucine. It is naturally produced in human body and small quantities can also be found in grapefruit, alfalfa, and catfish. Calcium HMB is used as a supplement because research has shown it to Increase sarcolemmal integrity via conversion to HMG-CoA and to enhance protein synthesis. It also helps in depression of protein degradation through inhibition of the ubiquitin pathway. Calcium HMB is proved to help muscle formulation so it is given to patients with HIV or AIDS and trauma victims with severe injuries. It is also used in treatment of cancer.

Creatine Monohydrate- Creatine is a naturally occurring compound that is formed in the various organs of the body, such as the pancreas, kidneys and the liver. Through the bloodstream it is transported to the muscles in the body. Creatine helps in building lean muscle mass and is extremely important to people who are into body building. Creatine monohydrate is a well known supplement that is used by a large number of athletes. Creatine monohydrate also has the ability to release the reserves of energy stored in the muscular tissues, thereby making it extremely important to athletes. Creatine, however, does have side effects that can cause cramps in the stomach, vomiting and dehydration, due to its ability to draw out water from the cells in the muscular tissue.

L-Arginine is one of the most common naturally occurring amino acids. In adults, arginine is deemed semi-essential, while for infants it is believed to be essential alpha amino acid. The functions of L-Arginine are multiple, it plays roles in the healing of wounds, immune function, hormone release, ammonia removal and cell division. It is also believed to be a precursor of nitric oxide, increasing the muscle mass, reduce body fat, decrease blood pressure and a dozen other functions, such as reducing the recuperation time after a serious injury, bone fractures and similar in particular. Since that L-Arginine can be produced by the human body, ingestion is conditionally not necessary, but sources include dairy products, meat such as beef and pork, poultry, but also vegetables like nuts, seeds, oatmeal, wheat flour and more.

L-Citrulline is an amino acid that helps in improving the flow of blood by changing into L-arginine and then nitric oxide. Nitric oxide helps to improve the mental and physical well-being of an individual by increasing blood circulation. Due to this action of the amino acid on the circulation of blood in the body, blood pressure problems are kept well under control. l-citrulline assists in the stimulation of growth enhancing hormones, helps in promoting the smooth functioning of the Urea Cycle, responsible for building up a part of the energy reserve of the body and in fortifying the immune system. A deficiency in this particular amino acid leads to fatigue- both mentally and physically as well as being one of the causes of sexual dysfunction.

L-Glycine- Glycine is an organic compound and one of the 20 amino acids deemed as building blocks for proteins. This proteinogenic amino acid is not only the smallest but also the only amino acid of the group that is not chiral, which means there is no enantiomer of glycine. With the prominent exception of collagen, which thirty five percent are glycine, most proteins contain only small amounts of it. As a solid, glycine is sweet tasting, crystalline and has no color. Despite being non-essential, because it can be biosynthesized from the amino acid serine, glycine has several important physiological functions, besides being a precursor to proteins. It is a biosynthetic intermediate of purines and the D-Aminolevulinic acid and it is an inhibitory neurotransmitter found in the central nervous system.

L-Ornithine is produced when the enzyme arginase reacts on L-arginine, whereby urea is being created. Consequentially, ornithine seems to be a vital part of the urea cycle, the disposal of ammonia and the removal of excess nitrogen. Although ornithine is not coded for by the DNA, which means that it is not a part of the protein synthesis processes. Despite this fact, the urea cycle plays a big part in arginine biosynthesis, which means that as an intermediate in a metabolic process, ornithine still retains vital importance. L-Ornithine is gaining on popularity as a nutritional supplement, in particular with the body building circles, where it is propagated as a muscle building and fat loss agent. It is furthermore stated that L-Ornithine may be initiating the release of growth hormones in the body.