Beta-Hydroxy-Beta-Methylbutyrate or Calcium HMB is a metabolite of the essential amino acid leucine. It is naturally produced in human body and small quantities can also be found in grapefruit, alfalfa, and catfish. Calcium HMB is used as a supplement because research has shown it to Increase sarcolemmal integrity via conversion to HMG-CoA and to enhance protein synthesis. It also helps in depression of protein degradation through inhibition of the ubiquitin pathway. Calcium HMB is proved to help muscle formulation so it is given to patients with HIV or AIDS and trauma victims with severe injuries. It is also used in treatment of cancer.

Creatine Monohydrate is composed of three amino acids I-glycine, I-arginine and I-methionine, Creatine Monohydrate is a metabolite used for supply of energy to the nerve cells and muscles in the human body. In recent times, it has become one of the most effective supplements for bodybuilding. It can help increase lean muscle mass within weeks and enhance the energy reserves in the muscles. Athletes use it for improving their overall performance as creatine is responsible for production of energy in the human body. You can take Creatine Monohydrate supplements in two ways. The first method is known as loading which includes the saturating or loading up of creatine in your muscles. The second method is a more gradual supplementation of Creatine Monohydrate. You can supplement with 5 to 15 grams per day.

L-Arginine is a very important amino acid that is required by the body for a number of functions. One of the main functions of L-Arginine is its ability to rid the body of ammonia, which is a waste product. It is also used in the manufacture of nitric oxide, which helps to relax the blood vessels, thereby increasing the flow of blood and reducing the chances of a heart attack. It is advised that supplements of L-Arginine should not be administered following a heart attack for fear that it could possibly aggravate cardiac shock. L-Arginine is being used in treating people with erectile dysfunction, as it relaxes the blood vessels surrounding the penis. It is also used in treating migraine and blood pressure.

L-Citrulline- Important in optimizing the blood flow process relative to the conversion of L-arginine onto nitric oxide. This in turn helps to ward off physical fatigue.

L-Glycine- Glycine is an organic compound and one of the 20 amino acids deemed as building blocks for proteins. This proteinogenic amino acid is not only the smallest but also the only amino acid of the group that is not chiral, which means there is no enantiomer of glycine. With the prominent exception of collagen, which thirty five percent are glycine, most proteins contain only small amounts of it. As a solid, glycine is sweet tasting, crystalline and has no color. Despite being non-essential, because it can be biosynthesized from the amino acid serine, glycine has several important physiological functions, besides being a precursor to proteins. It is a biosynthetic intermediate of purines and the D-Aminolevulinic acid and it is an inhibitory neurotransmitter found in the central nervous system.

L-Ornithine is produced when the enzyme arginase reacts on L-arginine, whereby urea is being created. Consequentially, ornithine seems to be a vital part of the urea cycle, the disposal of ammonia and the removal of excess nitrogen. Although ornithine is not coded for by the DNA, which means that it is not a part of the protein synthesis processes. Despite this fact, the urea cycle plays a big part in arginine biosynthesis, which means that as an intermediate in a metabolic process, ornithine still retains vital importance. L-Ornithine is gaining on popularity as a nutritional supplement, in particular with the body building circles, where it is propagated as a muscle building and fat loss agent. It is furthermore stated that L-Ornithine may be initiating the release of growth hormones in the body.